eLife4d
In vivo targeted and deterministic single-cell malignant transformation
Oncogene permanent activation in a single targeted cell of zebrafish together with a reprogramming factor transient activation is sufficient to induce the malignant transformation of the cell and ...
Frontiers26d
Exploring the impact of MiR-92a-3p on FOLFOX chemoresistance biomarker genes in colon cancer cell lines
Following this, 10 ng of cDNA was amplified by real-time qPCR in an AriaMx Real-time PCR System (Agilent Technologies, Santa Clara, CA, USA) using the TaqMan™ Universal Master Mix II kit (Cat. # ...
gut.bmj26d
Gut microbiota composition reflects disease severity and dysfunctional immune responses in patients with COVID-19
Each one-step RT-qPCR reaction contained 10 µL of extracted RNA, 4 µL TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Massachusetts, USA) in a final reaction volume of 20 µL. Primer and ...
Frontiers25d
Distinct roles for interleukin-23 receptor signaling in regulatory T cells in sporadic and inflammation-associated carcinogenesis
RT-qPCR was performed in technical duplicates with TaqMan™ probes (ThermoFisher, Massachusetts) and TaqMan™ Universal PCR Master Mix (ThermoFisher, Massachusetts). RT-qPCR results were analyzed by the ...
News Medical8d
Promega's GoTaq Probe 1-Step RT-qPCR System
The GoTaq ® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing ...
eLife26d
Mitochondrial adenine base editing of mouse somatic tissues via adeno-associated viral delivery
Quantification of viral genomes copy number and relative mtDNA content by quantitative real-time PCR Quantitative real-time PCR analyses were conducted in a QuantStudioTM 3 system (Thermo Fisher) ...