Biological cells invest much of their resources into the production of enzymes, which catalyze the conversion of substrates into products. An international team of bioinformaticians and biophysicists ...
Using Eppendorf BioSpectrometer for the determination of enzyme activity and substrate concentration
This article shows how enzymatic activity can be effectively determined by means of the Eppendorf BioSpectrometer. The preliminary measurements were carried out by utilizing the “single λ-continuous” ...
Contrary to what previously thought, enzymes move towards areas with less substrate. They display such behavior despite not having any thinking or sensing capabilities. Contrary to what previously ...
This article describes a simple method to determine the optimum pH for the activity of one enzyme in a coupled enzyme assay. Decoupling of the two-step reaction is achieved by heat denaturing the ...
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